plasmid backbone (Addgene inc)
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Addgene inc
plasmid backbone
Plasmid Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 241 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sgrna+cloning/sgRNA(MS2)+cloning+backbone+(Plasmid+%2361424)/pm41833778-84-8-16
Average 95 stars, based on 241 article reviews
Plasmid Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 241 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sgrna+cloning/sgRNA(MS2)+cloning+backbone+(Plasmid+%2361424)/pm41833778-84-8-16
Average 95 stars, based on 241 article reviews
plasmid backbone - by Bioz Stars,
2026-09
95/100 stars
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Cloning:Article Title: Chronic lysosome damage boosts interferon responses to Palbociclib through a mitochondrial signalling axis Article Snippet: .. Where Gateway cloning was performed this was as per standard protocols using BP clonase II and LR clonase II (Invitrogen). Article Title: Detecting Methylation Changes Induced by Prime Editing Article Snippet: .. Backbone plasmids used for pegRNA and Article Title: Med12 cooperates with multiple differentiation signals to facilitate efficient lineage transitions in embryonic stem cells. Article Snippet: .. Cell lines carrying PiggyBac transgenes were kept under appropriate selection to prevent transgene repression over passaging. Article Title: Residual Breast Cancer Cells Co-opt SOX5-driven Endochondral Ossification to Maintain Dormancy Article Snippet: LentiV_Cas9_puro (Addgene, Cat. #108100) was utilized to create Cas9-expressing MTB/TAN -derived primary tumor cells. .. For CRISPR-Cas9 studies, Article Title: Detecting Methylation Changes Induced by Prime Editing. Article Snippet: .. Backbone plasmids used for pegRNA and Article Title: Zinc-finger proteins with a co-opted capsid domain anchor nucleosomes over transposon sequences Article Snippet: To establish a HAP1 cell line with its endogenous ZNF24 gene tagged with an FKBP12 F36V degron, the cells were first sorted based on cellular sizes with FACSAria II (BD Biosciences) to enrich for haploid cells as previously described ( ). .. The repair template plasmid construction and Article Title: Selict-seq profiles genome-wide off-target effects in adenosine base editing. Article Snippet: HEK293T (ATCC, CRL-11268) and MCF7 (ATCC, HTB-22) cells were separately maintained in Dulbecco’s modified Eagle medium (DMEM) and Minimum Essential Medium (MEM) supplemented with 10% Fetal Bovine Serum (FBS) and 1% penicillin / streptomycin at 37 ◦C under 5% CO 2 . .. Plasmid construction and Plasmid Preparation:Article Title: Chronic lysosome damage boosts interferon responses to Palbociclib through a mitochondrial signalling axis Article Snippet: .. Where Gateway cloning was performed this was as per standard protocols using BP clonase II and LR clonase II (Invitrogen). Article Title: Med12 cooperates with multiple differentiation signals to facilitate efficient lineage transitions in embryonic stem cells. Article Snippet: .. Cell lines carrying PiggyBac transgenes were kept under appropriate selection to prevent transgene repression over passaging. Article Title: Residual Breast Cancer Cells Co-opt SOX5-driven Endochondral Ossification to Maintain Dormancy Article Snippet: LentiV_Cas9_puro (Addgene, Cat. #108100) was utilized to create Cas9-expressing MTB/TAN -derived primary tumor cells. .. For CRISPR-Cas9 studies, Article Title: Zinc-finger proteins with a co-opted capsid domain anchor nucleosomes over transposon sequences Article Snippet: To establish a HAP1 cell line with its endogenous ZNF24 gene tagged with an FKBP12 F36V degron, the cells were first sorted based on cellular sizes with FACSAria II (BD Biosciences) to enrich for haploid cells as previously described ( ). .. The repair template plasmid construction and Article Title: Selict-seq profiles genome-wide off-target effects in adenosine base editing. Article Snippet: HEK293T (ATCC, CRL-11268) and MCF7 (ATCC, HTB-22) cells were separately maintained in Dulbecco’s modified Eagle medium (DMEM) and Minimum Essential Medium (MEM) supplemented with 10% Fetal Bovine Serum (FBS) and 1% penicillin / streptomycin at 37 ◦C under 5% CO 2 . .. Plasmid construction and Selection:Article Title: Med12 cooperates with multiple differentiation signals to facilitate efficient lineage transitions in embryonic stem cells. Article Snippet: .. Cell lines carrying PiggyBac transgenes were kept under appropriate selection to prevent transgene repression over passaging. Passaging:Article Title: Med12 cooperates with multiple differentiation signals to facilitate efficient lineage transitions in embryonic stem cells. Article Snippet: .. Cell lines carrying PiggyBac transgenes were kept under appropriate selection to prevent transgene repression over passaging. Mutagenesis:Article Title: Med12 cooperates with multiple differentiation signals to facilitate efficient lineage transitions in embryonic stem cells. Article Snippet: .. Cell lines carrying PiggyBac transgenes were kept under appropriate selection to prevent transgene repression over passaging. CRISPR:Article Title: Med12 cooperates with multiple differentiation signals to facilitate efficient lineage transitions in embryonic stem cells. Article Snippet: .. Cell lines carrying PiggyBac transgenes were kept under appropriate selection to prevent transgene repression over passaging. Article Title: Residual Breast Cancer Cells Co-opt SOX5-driven Endochondral Ossification to Maintain Dormancy Article Snippet: LentiV_Cas9_puro (Addgene, Cat. #108100) was utilized to create Cas9-expressing MTB/TAN -derived primary tumor cells. .. For CRISPR-Cas9 studies, Clone Assay:Article Title: Med12 cooperates with multiple differentiation signals to facilitate efficient lineage transitions in embryonic stem cells. Article Snippet: .. Cell lines carrying PiggyBac transgenes were kept under appropriate selection to prevent transgene repression over passaging. other:Article Title: Loss of BPTF restores estrogen response and suppresses metastasis of mammary tumors. Article Snippet: Graphs were generated using GraphPad Prism software. Expressing:Article Title: Selict-seq profiles genome-wide off-target effects in adenosine base editing. Article Snippet: HEK293T (ATCC, CRL-11268) and MCF7 (ATCC, HTB-22) cells were separately maintained in Dulbecco’s modified Eagle medium (DMEM) and Minimum Essential Medium (MEM) supplemented with 10% Fetal Bovine Serum (FBS) and 1% penicillin / streptomycin at 37 ◦C under 5% CO 2 . .. Plasmid construction and Transfection:Article Title: Selict-seq profiles genome-wide off-target effects in adenosine base editing. Article Snippet: HEK293T (ATCC, CRL-11268) and MCF7 (ATCC, HTB-22) cells were separately maintained in Dulbecco’s modified Eagle medium (DMEM) and Minimum Essential Medium (MEM) supplemented with 10% Fetal Bovine Serum (FBS) and 1% penicillin / streptomycin at 37 ◦C under 5% CO 2 . .. Plasmid construction and |
